| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006255 |
| Chromosome: | chromosome 17 |
| Location: | 2882028 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718900 | FAP88 | Flagellar Associated Protein 88; (1 of 1) PTHR23237//PTHR23237:SF6 - NUCLEOLAR PROTEIN FAMILY A MEMBER 1 SNORNP PROTEIN GAR1 // H/ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 1 | outside_mRNA |
| Cre17.g718950 | RCA2 | (1 of 4) IPR003959//IPR027417 - ATPase, AAA-type, core // P-loop containing nucleoside triphosphate hydrolase; RuBisCO activase-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACTGGTGTCACGATGGCATCCCCGAGCGGAACGGCGCTTGGCAGAAG |
| Internal bar code: | CGGATGGTTGTTATATATGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1575 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCAGCAAAGTAGTCGCGC |
| Suggested primer 2: | GCTGACGGTTTGACACACAC |