| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006275 |
| Chromosome: | chromosome 10 |
| Location: | 1222763 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426292 | PYK6 | (1 of 3) PTHR11817//PTHR11817:SF4 - PYRUVATE KINASE // PYRUVATE KINASE; Pyruvate kinase 6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTGGTGTAAGCGTGCGTGTGTGTATGAGTGTTAGGGGGGGGGGGCA |
| Internal bar code: | TCGGAGATTTGAGGTCGTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAAAAGGGGGAGGGAAGG |
| Suggested primer 2: | CAACCCTGAACCCTCATCCC |