Insertion junction: CLIP2.006314_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.006314
Chromosome:chromosome 6
Location:2884187
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g272650 LHCA8 Light-harvesting protein of photosystem I; (1 of 3) K08911 - light-harvesting complex I chlorophyll a/b binding protein 5 (LHCA5) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGCAGCAGGGACAGAACGCATATAGATCACAGCCAGGTTACAGGAGGA
Internal bar code:CGCGGTAACTGAGGCAGCGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2675
LEAP-Seq percent confirming:4.91803
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTGTCCCTACGTGTTCC
Suggested primer 2:GAGGGTTTGCAGCAAAGTCG