Insertion junction: CLIP2.006314_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006314
Chromosome:chromosome 6
Location:2884191
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g272650 LHCA8 Light-harvesting protein of photosystem I; (1 of 3) K08911 - light-harvesting complex I chlorophyll a/b binding protein 5 (LHCA5) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTCTCGGGGCGACTGTCTTGGGTGGGAGAATCTGGAAGCTATGCAACG
Internal bar code:TCGTTACGTACGGTCTTTATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3510
LEAP-Seq percent confirming:63.2653
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGTTTGCAGCAAAGTCG
Suggested primer 2:TGCTTGTCCCTACGTGTTCC