| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.006319 |
| Chromosome: | chromosome 12 |
| Location: | 1776551 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485750 | CGL128 | (1 of 1) PTHR13420//PTHR13420:SF5 - UNCHARACTERIZED // UPF0235 PROTEIN; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTCGACTGGTTCCACCACTCGCACCACAACCACCGCTCGCGACCACA |
| Internal bar code: | CCTTAGACCTCGTTATATTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 28.2051 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTCGGCTCCCATATCCC |
| Suggested primer 2: | AAAGCAGTCGGATCGTGTGT |