| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006325 |
| Chromosome: | chromosome 17 |
| Location: | 2056625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711150 | FAD2a,FAD2 | Fatty acid desaturase, delta-12; (1 of 1) K10256 - omega-6 fatty acid desaturase (delta-12 desaturase) (FAD2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAAACCACTGCTACGTGCTTGCAGCTGCAGCAGCTGCCCCTGCTGCT |
| Internal bar code: | ATGATCTTGCAATATGAGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAACCAAACATCCCAAC |
| Suggested primer 2: | ATGTGACGTGCAGTTACCGT |