| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006330 |
| Chromosome: | chromosome 1 |
| Location: | 5739192 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041100 | HLM3 | (1 of 1) PTHR12197:SF75 - HISTONE-LYSINE N-METHYLTRANSFERASE ATXR2; Histone-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCAGCTCGTAATCGGCCTGACACCCCCACTCCAGACGTAACGGCCACC |
| Internal bar code: | TTATCTCGTGTAGTATACGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTTGAACAAAGGGCTGT |
| Suggested primer 2: | CAGATACGAAGCGTGCATGC |