| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006333 |
| Chromosome: | chromosome 6 |
| Location: | 4902191 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280650 | CGL59,Y3IP1 | Chloroplast Ycf3 interacting protein involved in Photosystem I biogensis | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATGCACGCTTCATCGCGTTCAACCGGGAAGGGCCTCCCACTCCAATC |
| Internal bar code: | AGTTTCAGGACATGGGCCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3042 |
| LEAP-Seq percent confirming: | 82.2581 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAAAAATGTCCGCGCAA |
| Suggested primer 2: | GAGGTGTGTGAGGGTCTTCG |