| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006356 |
| Chromosome: | chromosome 6 |
| Location: | 532962 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g252350 | CGL70 | (1 of 1) PTHR33102:SF13 - DVL17; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGCTCCTCTACCCCCCTCGGGCTCGGGCACCTAACCCCCCGAAGTCC |
| Internal bar code: | CGAGGTTTCAGAAACATCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACTACGGGGCCAGCAAAG |
| Suggested primer 2: | TGTAAACTTGGGTTTGCGCG |