| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006364 |
| Chromosome: | chromosome 7 |
| Location: | 1946931 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325752 | FAP258 | Flagellar Associated Protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCACTCCGCCGGGGCCGGCAGAAATAGAGCAGCAAGGGTGCGCATCA |
| Internal bar code: | TATCGGCGCGCGATGTCCTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2321 |
| LEAP-Seq percent confirming: | 60.2941 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGGGATGGAGTGAGTG |
| Suggested primer 2: | GCACAAGCGAACATGACGTT |