Insertion junction: CLIP2.006379_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.006379
Chromosome:chromosome 17
Location:3532755
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g724600 PAO2,TIC55-2 Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGGGCCGCACGCGAGCCAACCGCCAGCCGGGCCGCCGCTCGCTGCACC
Internal bar code:GAGACTCGTTGTTTAAATATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:954
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCGTGGTTTTGGAGTTG
Suggested primer 2:ATCCAGTGTTGCCCCTGATG