| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.006383 |
| Chromosome: | chromosome 4 |
| Location: | 1677899 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212500 | CGL42 | (1 of 6) PF09353 - Domain of unknown function (DUF1995) (DUF1995); Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCCTGTACAGCTATACGTCCACTCCCAAACTTGTCATTCTGCCAGGT |
| Internal bar code: | CATAAATAACATACAGCTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGAGGTAGGGGTTACAT |
| Suggested primer 2: | CCACACGACAGACTGACGAA |