| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006386 |
| Chromosome: | chromosome 9 |
| Location: | 2550813 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392700 | CGL39 | Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCAGCATCATGGCAAAGGCAGAATTCGAAGCGTGAGGGGCCGAAGTC |
| Internal bar code: | GGTTGTACCATTAGCATTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2289 |
| LEAP-Seq percent confirming: | 83.0769 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGTGGATGGGGCGATAG |
| Suggested primer 2: | CGGATTGAATGGAGCGCTTG |