| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006389 |
| Chromosome: | chromosome 1 |
| Location: | 5051752 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035100 | (1 of 1) 5.4.99.18 - 5-(carboxyamino)imidazole ribonucleotide mutase / N(5)-carboxyaminoimidazole ribonucleotide mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTGCTATCTTCGGCGGGGCGCATAACGGGGAATTCCCGTCGCAGAAC |
| Internal bar code: | AACCGTGCAACAACGGGGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1594 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAATGATGAGTGCAGCGG |
| Suggested primer 2: | TCGTATGGTGCTGCTTGTGT |