| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006401 |
| Chromosome: | chromosome 16 |
| Location: | 1932171 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656100 | (1 of 1) PF07444 - Ycf66 protein N-terminus (Ycf66_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGCGCATCTGCCCGCTTCGTGGGCAACGGTGCCGAGATCTGCTGATG |
| Internal bar code: | CCTTTTCACTTAAAGAGTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1116 |
| LEAP-Seq percent confirming: | 63.1579 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGATGGGGTTGCCGTAAT |
| Suggested primer 2: | TTCGCTAGTCCCTCTCTGCT |