| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.006409 |
| Chromosome: | chromosome 8 |
| Location: | 3166348 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376450 | CPLD36 | Conserved in the Plant Lineage and Diatoms; (1 of 1) PF12159 - Protein of unknown function (DUF3593) (DUF3593) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGTCACAGCGTCTCCCTTCGCCAGGTCACCTGGTCCAAACATGGCGC |
| Internal bar code: | CATGGGTCTTATGTGAGGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGGATCGAAGTCGGAG |
| Suggested primer 2: | ACCGCACATATCGATGGCAT |