Insertion junction: CLIP2.006413_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006413
Chromosome:chromosome 2
Location:2554545
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g092550 TPR1 (1 of 1) 6.2.1.4 - Succinate--CoA ligase (GDP-forming) / Succinyl-CoA synthetase (GDP-forming); Tetratricopeptide-repeat protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCATGGTCAAGATGATGAAATCCATGGATCCAGAGGCGCTGGCGGGCA
Internal bar code:CTGCTAATAGAGTTTATTATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3364
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCCTGCTTTCATACCCAC
Suggested primer 2:GATGGCGGCAGAGATGATGA