| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006416 |
| Chromosome: | chromosome 1 |
| Location: | 6337054 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044850 | (1 of 1) K17592 - sacsin (SACS) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGGCGGCGGCGGCGGCGGCATGGGCGGCACAGAGCTGCTGCAGGTC |
| Internal bar code: | TGTGTAGTTCCGTTTATAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 4.25532 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGTGTGGAGGTGTGAG |
| Suggested primer 2: | AGTCGTGACGTCTGGAGGTA |