Insertion junction: CLIP2.006419_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.006419
Chromosome:chromosome 3
Location:143046
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g143947 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGCTTCGTGCAGACACCCGATGACGAAGGCACTCACGAAGGGACAGA
Internal bar code:CAGCCTCTGGAGACAGTTTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:549
LEAP-Seq percent confirming:36.3636
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGACTATCACTCCGTGC
Suggested primer 2:GCACATCGCGATCAAGACAC