| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.006422 |
| Chromosome: | chromosome 2 |
| Location: | 8014154 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144850 | CGLD26b,CGLD26 | Conserved in the Green Lineage and Diatoms | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACACTATCTGAGGCAGCCTGTAGGACAGATGCCGTGTCAGCGCAGGC |
| Internal bar code: | TGCTTAGTGTGTTGTTGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1912 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAGGAAGCCGAAGCAGT |
| Suggested primer 2: | CCCCTGCTCATTCACCCAAT |