Insertion junction: CLIP2.006422_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.006422
Chromosome:chromosome 2
Location:8014157
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g144850 CGLD26b,CGLD26 Conserved in the Green Lineage and Diatoms CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGCTAGCGGCCATCGCCAAGCAGATCCTGCAAACAGATCCCGAGGCGC
Internal bar code:CCTTAACTTCTTGGTGAATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:875
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTGCTCATTCACCCAAT
Suggested primer 2:GAGAAGGAAGCCGAAGCAGT