Insertion junction: CLIP2.006428_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.006428
Chromosome:chromosome 16
Location:4760483
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g687900 LHCA7 Light-harvesting protein of photosystem I; (1 of 3) K08911 - light-harvesting complex I chlorophyll a/b binding protein 5 (LHCA5) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATACAAGGTGCCAGTATGATCAGGTATTGATTATCAATTTGCGATCATG
Internal bar code:AACGACATATCGACCTGGACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:78
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGGTGTGGATCTTCTGCG
Suggested primer 2:GCTGCTTGAAAGATAGCGCC