| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.006436 |
| Chromosome: | chromosome 3 |
| Location: | 3156784 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164900 | (1 of 1) PTHR24361//PTHR24361:SF159 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SERINE/THREONINE-PROTEIN KINASE OSR1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTATTCCCGCCGCCCCAAAACGACGGTCTCCTCCATCCCGCCACCCCTC |
| Internal bar code: | GGACTTTTTTAAAACGCCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1585 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGGCGGTTGTTGTTTAC |
| Suggested primer 2: | AATGGCGGATTCGTGGAACT |