Insertion junction: CLIP2.006442_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.006442
Chromosome:chromosome 3
Location:8421720
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g213201 OPR108,CCS2 Cytochrome c assembly facor 5'UTR
Cre03.g213313 (1 of 2) K00059 - 3-oxoacyl- (fabG) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGGTCTTCATGTCATGCGCAGGTCCCATGTACTGAACGTCAAAAAGCA
Internal bar code:CCGGGTGGGAATCGAGGGTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1236
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ACACTCCCTGTCCCTCTCTC
Suggested primer 2:TGGGTCTTTACACGGCTGAC