| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006464 |
| Chromosome: | chromosome 1 |
| Location: | 3105366 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019750 | (1 of 1) PTHR10871:SF4 - DNA TOPOISOMERASE 6 SUBUNIT B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCGGCGCATTTCCGCCTCCGCCCCCTTAAGTTACTCCGACTTGCACT |
| Internal bar code: | TGTTTCTCGGCAGAGGTCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGGTCCTACGACGACGC |
| Suggested primer 2: | CCTACATCCTGCGCTACCTG |