| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006471 |
| Chromosome: | chromosome 12 |
| Location: | 1636901 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACTTCTCGCCGCTGTCGGTCAACATGAGCAAATCAGTCAACCTGAGG |
| Internal bar code: | TCGAACTAGGGGACGCGTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2474 |
| LEAP-Seq percent confirming: | 74.0741 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTTCCCCATGAATGCCT |
| Suggested primer 2: | GCTGTCCTTGTCCTCGTTGA |