| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006481 |
| Chromosome: | chromosome 1 |
| Location: | 4717473 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032700 | GLK1 | (1 of 1) 2.7.1.2 - Glucokinase / Glucose kinase; Glucokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCTCCCTGCCTGGACAGTCTTCCCTTCTGGGCGTGGCAATGCTTTGGC |
| Internal bar code: | GATAGAGGGCGGGGCCGGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1443 |
| LEAP-Seq percent confirming: | 88.1356 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGCCTGATGACCAACGT |
| Suggested primer 2: | CGCTGGCATAACATGAACGG |