Insertion junction: CLIP2.006483_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.006483
Chromosome:chromosome 3
Location:3896730
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g170650 (1 of 1) IPR001849//IPR011993 - Pleckstrin homology domain // Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCAGGTGGGTGGGTGGGGAGGGGGCCGAGATTGGATGGGCGCGAGGAG
Internal bar code:TAGGGTACAAATAATGGGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:762
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGTACGGTGATAACAGCCGA
Suggested primer 2:CCGTGCTGCAAACTCAGAAC