| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006485 |
| Chromosome: | chromosome 2 |
| Location: | 7710549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146950 | DBR1 | RNA lariat debranching enzyme; (1 of 1) K18328 - lariat debranching enzyme [EC:3.1.-.-] (DBR1) | 5'UTR |
| Cre02.g147000 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGAGTGCAGACACGCCGAGCACCTCCTACTCGCGCGTAGAGTCGCCCG |
| Internal bar code: | ATACCTATCTAGGGTGTGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1086 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGAAGCACACCTGGTACT |
| Suggested primer 2: | CGTTGGCTAGGTGACAAGGT |