| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006515 |
| Chromosome: | chromosome 16 |
| Location: | 1950813 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656300 | DPR1 | (1 of 2) 1.17.1.8 - 4-hydroxy-tetrahydrodipicolinate reductase / Dihydrodipicolinate reductase; dihydropicolinate (DAP) reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAAGGCCGAGTACCCCGGCATGATGATTGTGGACTACACATTGGCCC |
| Internal bar code: | GGTTATAAGGCGAGTGAGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3578 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCGATTGAACTGTCCA |
| Suggested primer 2: | CTCCTGTACTGGCAAGGTGG |