| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006517 |
| Chromosome: | chromosome 2 |
| Location: | 4728957 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGACGGGTGCCCCACGGGTGCCCCCAGGGCCCAGGCCCAGGCGTCGAA |
| Internal bar code: | ACAGTCGATAGCGCCATGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3343 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGAAGGAGAAGGAGGAC |
| Suggested primer 2: | TAAGGTCATCACGGCCCCTA |