| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006521 |
| Chromosome: | chromosome 12 |
| Location: | 5347000 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGAGCGTTTGGATGATTTAATGAAGGGAAACATGCAGGCACGCCATC |
| Internal bar code: | GGAGTGAGTGCCTATTTCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4289 |
| LEAP-Seq percent confirming: | 78.3784 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGAGGGCTGGGGACATGA |
| Suggested primer 2: | AAGAAGCTGGCCTTGGACTC |