| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006537 |
| Chromosome: | chromosome 7 |
| Location: | 5111211 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348100 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTTTGAGTGCAACTATCACCGCAAATCCGACGTGCTTGCAAGCCTTTG |
| Internal bar code: | CTGACTTCCATTCAATCCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2121 |
| LEAP-Seq percent confirming: | 82.6531 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTGCTTGTCCCACGTCA |
| Suggested primer 2: | CACTCCAGCAAAGTAGCGGA |