| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006539 |
| Chromosome: | chromosome 8 |
| Location: | 850883 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361250 | (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCCTTCCCGCCCGGCACCTGCTTCGCCTCCGCCTTCCACGTCTACAC |
| Internal bar code: | CTACAAGCGCGAATGGGTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTGACCCATCCACCCAC |
| Suggested primer 2: | AGGGCTAAGGGAAGGAGAGG |