| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006551 |
| Chromosome: | chromosome 3 |
| Location: | 5246760 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182150 | TEF8 | (1 of 1) PTHR30373:SF2 - UPF0603 PROTEIN YGCG; Thylakoid Lumen Protein 18.3 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCGCTGCTCCTCTCGTCCGCCGCCGTGCCGTGCAGATATGCTGCAAG |
| Internal bar code: | AGAAGACGATCACGTAATTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2473 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 119 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCCACGTGCTGATGATA |
| Suggested primer 2: | CGGTGTGTGCCGTGAATTTT |