| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006562 |
| Chromosome: | chromosome 2 |
| Location: | 3200904 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096800 | (1 of 2) PF02410 - Ribosomal silencing factor during starvation (RsfS) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGCGGGTGTGTGGGTCGTGTGGGTGGCGTTGGGTGGGGGGTGGGGGT |
| Internal bar code: | CTACGTGGTGCCTGTAATGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 37.2093 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCTGAGCACTACCAGCG |
| Suggested primer 2: | TGTTGTGTCCGAAGCCTCTC |