| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006582 |
| Chromosome: | chromosome 3 |
| Location: | 4948235 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGACGGGTAGATGCTACCGGTATACACGCCCAAGACTTTATTCCCAT |
| Internal bar code: | GTTTGATGAATTCCAGTATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3753 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGCGTTAGAGCATAGTC |
| Suggested primer 2: | ACGGAGTGAGTCATTCGTCG |