Insertion junction: CLIP2.006590_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.006590
Chromosome:chromosome 6
Location:7040274
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g297400 (1 of 1) 1.3.1.74 - 2-alkenal reductase (NAD(P)(+)) / NADPH:2-alkenal alpha,beta-hydrogenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCACAGGTGTGTCTGGTCGTGTCCGGCCCAAGTCGGGGCCCTTCCTT
Internal bar code:GTCTCTTCTTGGGAATTTGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:586
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAAGCAAACCAAACCGAG
Suggested primer 2:TGAGTCGTGGACAAGCTGTC