| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006596 |
| Chromosome: | chromosome 13 |
| Location: | 1284530 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570900 | MSRA1 | (1 of 2) PTHR10173:SF6 - PEPTIDE METHIONINE SULFOXIDE REDUCTASE; Peptide methionine-S-sulfoxide reductase, msrA-type | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGACCCTTCCGTGCGGCCGTGCAGGAGGCTTCGTTCGCGAAGCTTCC |
| Internal bar code: | AACGTTAGTCAGTAGTTCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3500 |
| LEAP-Seq percent confirming: | 67.3077 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTCCTCGTACGAGATG |
| Suggested primer 2: | GTATGGCGATCGGACTGAGG |