Insertion junction: CLIP2.006608_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006608
Chromosome:chromosome 3
Location:636659
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145587 CPLD1 Conserved in the Plant Lineage and Diatoms; (1 of 4) PF04134 - Protein of unknown function, DUF393 (DUF393) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGCCCACACGTCGTACACCTTGTTAGCCAGGGTCAACACAATGGGAAT
Internal bar code:GACGGAGGCCGTGGACGGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1996
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTTTGCACTTGGCCATGA
Suggested primer 2:GAAGGGGCTTTACTGGTGCT