Insertion junction: CLIP2.006609_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.006609
Chromosome:chromosome 6
Location:2658007
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g270400 GT90-6,GT90F6 GT90 family protein 6; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAAAGTCGCTCAGCTTCGGCTGGTAGGTAAACGCAGCTGCCAGGCGCT
Internal bar code:TTGGGCGGCAGAGCTGGTATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1193
LEAP-Seq percent confirming:39.3939
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCTCCTCGCTTCCATCT
Suggested primer 2:CCAAGGAGTTGTGCTCGTCT