Insertion junction: CLIP2.006616_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.006616
Chromosome:chromosome 10
Location:2360032
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g434750 AAI1 (1 of 1) 1.1.1.86 - Ketol-acid reductoisomerase (NADP(+)) / Dihydroxyisovalerate dehydrogenase (isomerizing); Acetohydroxy acid isomeroreductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCCCATCTCCCGCACCATCTCGACCAAGGGCATGCTGTCCGTCTACAA
Internal bar code:GGCTTAATCATCGTATCTTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2835
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGGGGTTAGGGGGTAAGC
Suggested primer 2:CAGACGCGCAAGATTGAGTG