| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006627 |
| Chromosome: | chromosome 16 |
| Location: | 4531412 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671500 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAAGGGGAGGCGGTAAGCTGACAGGAGAGGCGGGCACAACTGGCGAGT |
| Internal bar code: | GCGGTGGCGAATTATGTAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1092 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATTGCTGCGGCTACATG |
| Suggested primer 2: | ACCCCCACTATCACTACCCC |