| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006628 |
| Chromosome: | chromosome 16 |
| Location: | 5816207 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679950 | RFC3 | DNA replication factor C complex subunit 3; (1 of 1) PTHR11669:SF1 - REPLICATION FACTOR C SUBUNIT 3 | 3'UTR |
| Cre16.g680000 | ATP5 | (1 of 1) K02137 - F-type H+-transporting ATPase subunit O (ATPeF0O, ATP5O, ATP5); Mitochondrial ATP synthase subunit 5, OSCP subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTACCCGCTTGAGGACTGCGCGTGGCATGTGCTGAGTGCCTGCATGTA |
| Internal bar code: | TGGTGACTATCAGAGTATCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1856 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCTGAAAGTGCTTGCGC |
| Suggested primer 2: | GACAAGTTGTTGGCTGGCTG |