Insertion junction: CLIP2.006630_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.006630
Chromosome:chromosome 4
Location:3763336
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g229494 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGGCACGCCTCCTTCACAGTTTGGCCCCCCCCCTCCTCACCGTCCTTC
Internal bar code:GAAAAGGACTTGAACTTCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:182
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCTGTCGCCATTGTTTG
Suggested primer 2:CCCTTGTCAGCAGGTAAGCA