Insertion junction: CLIP2.006634_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.006634
Chromosome:chromosome 9
Location:3121216
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394251 PF16 Central pair associated protein; (1 of 1) PF00514//PF13513 - Armadillo/beta-catenin-like repeat (Arm) // HEAT-like repeat (HEAT_EZ) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTAGGTGCGGAGCATACGATGCCATTCCGTGTGCATGCAGGGGGGTCG
Internal bar code:CGGGGACGAATTGTGTCGGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2083
LEAP-Seq percent confirming:96.2963
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTAGTTACCTGCCTCGAG
Suggested primer 2:CCGCATGCAGGAGTAAATGC