Insertion junction: CLIP2.006637_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.006637
Chromosome:chromosome 17
Location:4536380
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g731571 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCGTGCCCTGCATGCGGTGGTCCATGTCACGAGGGGGGGGTCGGGCAA
Internal bar code:GGGATATTAGGGGTAACAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:10679
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CACACGTCGACCTTTCCTGA
Suggested primer 2:CCGACACAACAAAGCCACAG