| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006640 |
| Chromosome: | chromosome 6 |
| Location: | 2856591 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272300 | CPLD57 | (1 of 1) PTHR23029:SF45 - PHOSPHOGLYCERATE MUTASE FAMILY PROTEIN; Phosphoglycerate mutase family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGCTGGGCCACAGCCAACAACCATCGCTGCAGGCGCCCAGGGACTC |
| Internal bar code: | CTGAGAGATTGTAGTTTCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2182 |
| LEAP-Seq percent confirming: | 55.8824 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAAAACTGTCACCGTGC |
| Suggested primer 2: | AGTGGTGTCGCAAAAATGCC |