Insertion junction: CLIP2.006659_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006659
Chromosome:chromosome 1
Location:3398881
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g021950 (1 of 1) PF08393//PF12777//PF12781 - Dynein heavy chain, N-terminal region 2 (DHC_N2) // Microtubule-binding stalk of dynein motor (MT) // ATP-binding dynein motor region D5 (AAA_9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGCCACCTCCACCAGCCCCTGGTCCGGCTCCCAGTAGTGCCGCACGTT
Internal bar code:TCGACGTGGTGTGACTGTATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:439
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TACGCGTGTGATCTCGTCTG
Suggested primer 2:GTTTGCGCTAGGAGGTGCTA