| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006672 |
| Chromosome: | chromosome 3 |
| Location: | 5197640 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181500 | DPE1,STA11 | Disproportionating enzyme 1; (1 of 1) PTHR32438:SF5 - 4-ALPHA-GLUCANOTRANSFERASE DPE1, CHLOROPLASTIC/AMYLOPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGTTGGAGGAGTTTGCACGGCACCTCACTGGTGGTCCAGGCGTCTT |
| Internal bar code: | TCTACAGGGTGAGGCATGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2924 |
| LEAP-Seq percent confirming: | 68.4211 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCGTGTCATGAGCACATA |
| Suggested primer 2: | CCTTGTACCGTTCCGTGTCA |